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RESEARCH
Improving the quality of 3DEM biological information by the use of new advanced reconstruction algorithms
Introduction to the problem
Size range of particles studied with TEM
X ray transform inversion
Central Section Theorem
Series expansion algorithms
ARTk
Angular assignment problem
Angular assignment algorithm
Coding images as vectors
Projection matching algorithm
Wavelet space
Wavelet space for images
Projection matching algorithm
Angular assignment algorithm
Euler angles
3DEM reconstruction optimization
Blob and grid optimization
Optimization of the reconstruction space
Free parameter optimization
Normalization study
Optimization of the projection order
CTF detection and correction
Theoretical model
Spectral estimation
Theoretical CTF adjustment to that estimation
CTF correction using the adjusted theoretical model
A priori knowledge
Symmetry
Volumetric restrictions
Surface
Total mass
Results with single particles
2D Crystal reconstruction
What is the resolution achieved?
Spectral SNR 3D
Conclusions
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