What is PeppeR ?

PeppeR is the Java-based, graphical DAS client, intended for visualization of hybrid models (3D-EM volume maps plus atomic resolution structures from fitting experiments) together with their annotations.

Launcher PeppeR

PeppeR implements DASx3DEM, an extension of the DAS protocol that allows sharing annotations about hybrid models. This annotation system has been built on the basis of the EMDB, which stores Three-dimensional Electron Microscopy (3D-EM) volumes, PDB, which houses atomic coordinates, and UniProt (for protein sequences) databases. In this way, annotations for sequences, atomic coordinates, and 3D-EM volumes are collected and displayed through a single graphical visualization client. Thus, users have an integrated view of all the annotations together with the whole macromolecule (3D-EM map coming from EMDB), the atomic resolution structures fitted into it (coordinates coming from PDB) and the sequences corresponding to each of the structures (from UniProt).

Downloads

Download current or previous versions of PeppeR.

Publications

When citing PeppeR please reference:


Integrating electron microscopy information into existing Distributed Annotation Systems.
J R Macías, N Jiménez-Lozano, J M Carazo (2007)
Journal of structural biology 158 (2) p. 205-13
PMID: 17400476; doi: 10.1016/j.jsb.2007.02.004


Your can also find PeppeR references here:

  • Moreno BG, Peypoch XM. GenExp: An interactive web-based genomic DAS client with client-side data rendering. PLoS ONE. 2011;6(7). PMID: 21750706; PMCID: PMC3130039; doi: 10.1371/journal.pone.0021270
  • Jiménez-Lozano N, Segura J, Macías JR, Vega J, Carazo JM. aGEM: An integrative system for analyzing spatial-temporal gene-expression information. Bioinformatics. 2009;25(19):2566-72.PMID: 19592395; doi: 10.1093/bioinformatics/btp422
  • Martínez Barrio Á, Lagercrantz E, Sperber GO, Blomberg J, Bongcam-Rudloff E. Annotation and visualization of endogenous retroviral sequences using the distributed annotation system (DAS) and eBioX. BMC Bioinformatics. 2009;10(SUPPL. 6). PMCID:2697641; doi: 10.1186/1471-2105-10-S6-S18
  • Blankenburg H, Finn RD, Prlić A, Jenkinson AM, Ramírez F, Emig D, Schelhorn S-, Búch J, Lengauer T, Albrecht M. DASMI: Exchanging, annotating and assessing molecular interaction data. Bioinformatics. 2009;25(10):1321-8. PMID: 19420069; doi: 10.1093/bioinformatics/btp142
  • Jenkinson AM, Albrecht M, Birney E, Blankenburg H, Down T, Finn RD, Hermjakob H, Hubbard TJP, Jimenez RC, Jones P, Kähäri A, Kulesha E, Macías JR, Reeves GA, Prlić A. Integrating biological data - the distributed annotation system. BMC Bioinformatics. 2008;9(SUPPL. 8). PMID: 18673527; doi: 10.1186/1471-2105-9-S8-S3
  • Mons B, Ashburner M, Chichester C, Van Mulligen E, Weeber M, den Dunnen J, Van Ommen G-, Musen M, Cockerill M, Hermjakob H, Mons A, Packer A, Pacheco R, Lewis S, Berkeley A, Melton W, Barris N, Wales J, Meijssen G, Moeller E, Roes PJ, Borner K, Bairoch A. Calling on a million minds for community annotation in WikiProteins. Genome Biol. 2008;9(5). PMID: 18507872; doi: 10.1186/gb-2008-9-5-r89
  • Goddard TD, Ferrin TE. Visualization software for molecular assemblies. Curr Opin Struct Biol. 2007;17(5):587-95. PMID: 17728125; doi: 10.1016/j.sbi.2007.06.008
  • Prlić A, Down TA, Kulesha E, Finn RD, Kähäri A, Hubbard TJP. Integrating sequence and structural biology with DAS. BMC Bioinformatics. 2007;8:333. PMID:17850653;doi: 10.1186/1471-2105-8-333

Disclaimer: PeppeR requires that you accept the security certificate in order to access the local file system. Some files are written or created during the execution of the code, simply as a cache. Those files can be safely cleaned when shutting down the application. This software comes with no warranty and by accepting the certificate you agree that the authors of the code (DAS Team) are not responsible for any damage incurred.