Welcome to FluoJ
Fluorescence microscopy has become an essential technique in cell and molecular biology, increasingly used to characterize different biological processes. Here we present FluoJ, developed as a computer workbench to partially automate fluorescence analysis workflows easying the analysis of a large number of events. We start from the consideration that image processing tasks are, indeed, different for each sample, but that there is, usually, a common set of core steps in each procedure, such as image preprocessing, segmentation, specimen characterization, classification and quantification. Within FluoJ the user can automate these steps in an easy manner for many workflows. The software has been used in a variety of different studies, improving image analysis quantification and minimizing user intervention.
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